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KMID : 0861020110260010133
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Korea Journal of Herbology
2011 Volume.26 No. 1 p.133 ~ p.138
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The Antioxidant Activity of Sesami Semen Nigrum on Leydig TM3 cells
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Chang Mun-Seog
Chung Kyu-Jin
Park Seong-Kyu
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Abstract
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Objectives:The purpose of this study was to estimate the antioxidant activity of Sesami Semen Nigrum extract (SSN) on mouse Leydig cells, TM3.
Methods: Cell viability assays were performed. The protective effects of SSN against hydrogen peroxide-induced oxidative stress in Leydig cells were examined by measuring cell viability. Lipid peroxidation levels and antioxidant enzyme concentrations such as SOD and catalase were measured.
Results: Cell viability of Leydig cells increased with SSN concentration. Cell viability of Leydig cells was 136.66% when SSN concentration was 50㎍/㎖. Cell viability of the hydrogen peroxide group was statistically decreased (p<0.01) compared with the control group. Antioxidant effect of SSN was measured and the protective effect of SSN concentration were 5, 10, 50㎍/㎖. LPO were decreased significantly at 5, 50㎍/㎖ of SSN concentrations. SOD activity was increased at 1, 10, 50㎍/㎖ of SSN concentrations. Catalase activity was significantly increased at 123.7, 133.3 and 131.9 units/mg protein when SSN concentrations were 5, 10 and 50㎍/㎖, respectively.
Conclusions : In conclusion, Sesami Semen Nigrum extract has antioxidant activities in Leydig cells against oxidative stress.
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KEYWORD
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Sesami Semen Nigrum extract (SSN), Leydig cells, Cell viability, LPO, SOD, Catalase
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